Fig. 2
Physioxia enhanced ASC proliferation and migration through ROS upregulation. a The proliferation of P-ASCs and H-ASCs measured by WST-8 and cell doubling curves. b and d P-ASCs were treated with 100 μM BHA to inhibit ROS, as detected by flow cytometry. The relative MFI was quantified by the ratio of the MFI for P-ASCs and P-ASCs (BHA) to that of H-ASCs. c The proliferation of P-ASCs, H-ASCs and P-ASCs (BHA) measured by WST-8 and cell doubling curves. e Transwell assays were used for determining cell migration, and the migrated cells were stained by 0.1% crystal violet. f The crystal violet in migrated cells was extracted by 10% acetic acid, and the optical density values were determined. The cell doubling curve was produced by dividing the cell number by 104 and then transforming the values to log2. Data are presented as the mean ± SD, *P < 0.05, **P < 0.01, Student’s t tests, scale bar = 100 μm. ASCs adipose-derived stem cells, BHA butylated hydroxyanisole, H-ASCs hyperoxia ASCs, MFI mean fluorescence intensity, P-ASCs physioxia ASCs, ROS reactive oxygen species