Fig. 4
Physioxia promoted angiogenic ability of ASCs. ASCs (2 × 104) were seeded onto 96-well plates coated with 50 μL of Matrigel and cultured for 6 h. a Mesh-like structures resulting from tube formation assay. b, c and d Total mesh, branching length, and junction values per field of view were quantified by ImageJ. Five fields were quantified. e Expression levels of mRNA encoding VEGF, VEGFR2, and vWF as measured by qRT-PCR. Data are presented as the mean ± SD, *P < 0.05 (P-ASCs/H-ASCs), **P < 0.01 (P-ASCs/H-ASCs), Student’s t tests, n = 3, scale bar = 100 μm. ASCs adipose-derived stem cells, H-ASCs hyperoxia ASCs, P-ASCs physioxia ASCs, qRT-PCR quantitative real-time polymerase chain reaction, VEGF vascular endothelial growth factor, VEGFR2 vascular endothelial growth factor receptor 2, vWF von Willebrand factor