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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Physioxia: a more effective approach for culturing human adipose-derived stem cells for cell transplantation

Fig. 6

Variations in mitochondrial and pH metabolism of P-ASCs. a and b Fluorescent images and flow cytometry results of mitochondrial mass determined by staining ASCs with NAO; the relative MFI was determined as the MFI of P-ASCs versus that of H-ASCs. c Expression of HIF-1 target genes evaluated by qRT-PCR. d Extracellular lactate concentration of P-ASCs and H-ASCs. e Cells cultured in the acidic model for 24 h were stained with BCECF-AM to determine the intracellular pH. Data are presented as the mean ± SD, *P < 0.05 (P-ASCs/H-ASCs), **P < 0.01 (P-ASCs/H-ASCs), Student’s t tests, n = 3, scale bar = 100 μm. ASCs adipose-derived stem cells, BCECF-AM 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethyl ester, BNIP3 BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, mitophagy regulator, COX4I1 cytochrome c oxidase subunit 4 isoform 2, metabolic enzyme, COX4I2 cytochrome c oxidase subunit 4 isoform 2, metabolic enzyme, H-ASCs hyperoxia ASCs, LDHA lactate dehydrogenase A, glycolysis, MCT4 monocarboxylate transporter 4, lactate discharge, NAO nonyl acridine orange, NHE2 sodium-hydrogen exchanger 2, H+ discharge, NHE3 sodium-hydrogen exchanger 3, H+ discharge, P-ASCs physioxia ASCs, PDK1 pyruvate dehydrogenase kinase 1, inactivating pyruvate dehydrogenase

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