Fig. 7

Increased glucose uptake and reserve of P-ASCs. a and b Fluorescent images and flow cytometry results of glucose uptake in ASCs determined by staining with 2-NBDG; the relative MFI was determined by MFI of P-ASCs versus that of H-ASCs. Scale bar = 100 μm. c Intracellular glycogen detected by PAS staining; the PAS⁺ area was calculated as the PAS⁺ area versus the total image area. Three fields were quantified. Scale bar = 50 μm. d Expression of HIF-1 target genes evaluated by qRT-PCR. Data are presented as the mean ± SD, *P < 0.05 (P-ASCs/H-ASCs), **P < 0.01 (P-ASCs/H-ASCs), Student’s t tests, n = 3. 2-NBDG 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose, ASCs adipose-derived stem cells, GLUT1 glucose transporter 1, glucose uptake, GLUT3 glucose transporter 3, glucose uptake, GYS1 glycogen synthase 1, glycogen synthesis, H-ASCs hyperoxia ASCs. PAS periodic acid-Schiff, P-ASCs physioxia ASCs, PGM phosphoglucomutase, glycogen synthesis, PYGL liver isoform of glycogen phosphorylase, glycogen breakdown