Fig. 3
From: Melatonin rescued interleukin 1β-impaired chondrogenesis of human mesenchymal stem cells
MLT reversed IL-1β-inhibited COL2A1 expression at both mRNA and protein levels. qPCR and IHC analysis assessed COL2A1 expressions at 7, 14 and 21 days. mRNA expression of COL2A1 measured by qPCR and normalized to GAPDH. Relative expression levels of gene on 7 (b), 14 (d), and 21 (f) days representative of three independent experiments. aP < 0.05 versus control group; bP < 0.05 versus IL-1β group. IHC staining of collagen type II performed after 7 (a), 14 (c), and 21 (e) days of differentiation. Scale bars: 0.1 mm. IL-1β interleukin-1β, MLT melatonin