Fig. 6

MLT downregulated IL-1β-enhanced phosphorylation of P65 and IκBα in NF-κB pathway. Immunoblotting (a) conducted to detect expression and total and phosphorylated P65 and IκBα to reflect activation of NF-κB signaling. Phospho-P65, P65, phospho-IκBα, and IκBα protein levels established (b). Ratio of relative protein expression of p-P65 to relative protein expression of P65 (p-P65/P65) (c), and ratio of relative protein expression of p-IκBα to relative protein expression of IκBα (p-IκBα/IκBα) examined (d). ^aP < 0.05 versus control group; ^bP < 0.05 versus IL-1β group. IL-1β interleukin-1β, MLT melatonin