Table 1 All primers for teal-time RT-PCR assay
From: Melatonin rescued interleukin 1β-impaired chondrogenesis of human mesenchymal stem cells
Gene | Primer sequence |
|---|---|
COL2A1 | Sense: 5′-GGCAATAGCAGGTTCACGTACA-3′ Antisense: 5′-CGATAACAGTCTTGCCCCACTT-3′ |
ACAN | Sense: 5′-TGCATTCCACGAAGCTAACCTT-3′ Antisense: 5′-GACGCCTCGCCTTCTTGAA-3′ |
SOX9 | Sense: 5′-AGCGAACGCACATCAAGAC-3′ Antisense: 5′-GCTGTAGTGTGGGAGGTTGAA-3′ |
COL10A1 | Sense: 5′-CAAGGCACCATCTCCAGGAA-3′ Antisense: 5 ′-AAAGGGTATTTGTGGCAGCATATT-3′ |
MMP9 | Sense: 5′-TGTACCGCTATGGTTACACTCG-3′ Antisense: 5 ′-GGCAGGGACAGTTGCTTCT-3′ |
MMP13 | Sense: 5′-CCAGACTTCACGATGGCATTG-3′ Antisense: 5′-GGCATCTCCTCCATAATTTGGC-3′ |
ADAMTS4 | Sense: 5′-GAGGAGGAGATCGTGTTTCCA-3′ Antisense: 5 ′-CCAGCTCTAGTAGCAGCGTC-3′ |
GAPDH | Sense: 5′-AGAAAAACCTGCCAAATATGATGAC-3′ Antisense: 5 ′-TGGGTGTCGCTGTTGAAGTC-3′ |