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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: miR-10a rejuvenates aged human mesenchymal stem cells and improves heart function after myocardial infarction through KLF4

Fig. 1

Cell apoptosis increased in old hBM-MSCs under hypoxia conditions. Young (Y) and old (O) hBM-MSCs cultured for 72 h under hypoxia conditions. a Cell apoptosis assayed by TUNEL staining. Percentage of apoptotic cells (TUNEL+) quantified in Y and O hBM-MSCs. b Cell survival evaluated in Y and O hBM-MSCs c Protein expression of MCL1 and PUMA evaluated by western blot analysis in Y and O hBM-MSCs. d Ratio of Bax/BCL2 quantified in Y and O hBM-MSCs. e Protein expression of cleaved caspase-3 and inhibitor of caspase-activated DNase (ICAD) assayed in Y and O hBM-MSCs. f Caspase-3 activity measured in Y and O hBM-MSCs. Expression of (g) miR-10a and (h) KLF4 compared in Y and O hBM-MSCs. n = 6/group. Mean ± SD. *P < 0.05. DAPI 4′,6-diamidino-2-phenylindole, KLF4 Krüpple-like factor 4, TUNEL terminal deoxynucleotidyl transferase dUTP nick end labeling, RQ relative quantity, RFU relative fluorescence units

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