Fig. 6
Implantation of miR-10a-overexpressed or KLF4-downregulated old hBM-MSCs into ischemic area of mouse hearts improved cardiac function after MI. miR-10a-overexpressed or KLF4-downregulated old hBM-MSCs (3 × 105 cells/mouse) implanted into infarcted mouse hearts. Cardiac function determined by echocardiography in mice that received implantation of control medium (Media), control vector-transduced young hBM-MSCs (Y-c), control vector-transduced old hBM-MSCs (O-c), miR-10a-overexpressed old hBM-MSCs (O-10a), or KLF4-inhibited old hBM-MSCs (O-antiKLF4) into border region immediately following MI. Cardiac function measured by echocardiography at baseline (before MI, 0 day), 14 and 28 days after MI in all groups. a Representative M-mode echocardiographic images. b Fractional shortening. c Ejection fraction. d Left ventricular internal end-systolic dimension (LVIDs). e Left ventricular internal end-diastolic dimension (LVIDd). f Each panel shows one representative whole sectioned heart (from base to apex) at 28 days after MI from five individual groups (Media, Y-c, O-c, O-10a, or O-antiKLF4) to show scar areas (arrows) (g) and scar size thickness (h). i Representative images of Masson Trichrome’s staining at 28 days after MI. j Viable myocardium (identified as red with Trichrome’s staining) in ischemia zone quantified and expressed as percentage of total infarct area. n = 6/group. Mean ± SD. *P < 0.05, Y-c vs other groups; #P < 0.05, O-c vs other groups. LV left ventricle, MI myocardial infarction