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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: miR-10a rejuvenates aged human mesenchymal stem cells and improves heart function after myocardial infarction through KLF4

Fig. 8

miR-10a-upregulated or KLF4-downregulated hBM-MSCs increased mice heart angiogenesis after MI. miR-10a-overexpressed or KLF4-downregulated old hBM-MSCs (3 × 105 cells/mouse) implanted into infarcted mouse hearts. Angiogenesis and expression of angiogenic factors determined in mice that received implantation of control vector-transduced young hBM-MSCs (Y-c), control vector-transduced old hBM-MSCs (O-c), miR-10a-overexpressed old hBM-MSCs (O-10a), or KLF4-inhibited old hBM-MSCs (O-antiKLF4) into border region immediately following MI. Capillary and arteriole densities quantified by isolectin stain (a, b) and α-smooth muscle actin (α-SMA) stain respectively at 3 and 7 days (c, d) post MI. e, f VEGF and SDF protein expression evaluated by western blot analysis from border region of infarcted mouse hearts implanted with Y-c, O-c, O-10a, and O-antiKLF4 hBM-MSCs respectively. g, h VEGF and SDF protein expression evaluated by western blot analysis in Y-c, O-c, O-10a, and O-antiKLF4 hBM-MSCs. i VEGF and SDF protein in cell culture supernatant of Y-c, O-c, O-10a, and O-antiKLF4 hBM-MSCs assayed by ELISA. n = 6/group. Mean ± SD. *P < 0.05, Y-c vs other groups; #P < 0.05, O-c vs other groups. DAPI 4′,6-diamidino-2-phenylindole, hBM-MSC human mesenchymal stem cell, KLF4 Krüpple-like factor 4, MI myocardial infarction, SDF stromal cell-derived factor, VEGF vascular endothelial growth factor

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