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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients

Fig. 2

iPSC-MSCs enhanced Treg cell activation in unstimulated PBMCs. Flow cytometric analysis of CD25+CD69+ cells in CD4+ cells (a) and quantification of CD4+CD25+CD69+ subset percentage in CD4+ subpopulation (b) (n = 6). Flow cytometric analysis of CD4+CD69+ cells in CD4+CD25+ cells (c) and quantification of CD4+CD25+CD69+ subset percentages in CD4+CD25+ subpopulation (d) (n = 6). Flow cytometric analysis of CD4+Foxp3+ cells in PBMCs (e) and quantification of Foxp3+CD4+ Treg cells in quiescent CD4+ T cells (f) (n = 6). Flow cytometric analysis of CD69+Foxp3+ cells in CD4+ cells (g) and quantification of CD69+Foxp3+CD4+ Treg cells in quiescent CD4+ T cells (h) under different culture conditions. CD3+ T cells first gated (n = 6). i Representative histograms of CFSE-stained PBMC proliferation when cocultured with different conditioned Treg cells. CD3+ T cells first gated. j Statistical analysis of CFSE-stained CD3+ T-cell proliferation when cocultured with different conditioned Treg cells (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA and Dunnett T3 test for multiple comparisons for (b), (f), (h), and by Kruskal–Wallis rank-sum test followed by Mann–Whitney U test for two-group comparisons for (d), (j). AR allergic rhinitis, BM-MSC bone marrow-derived mesenchymal stem cell, CFSE carboxyfluorescein succinimidyl ester, iPSC-MSC induced pluripotent stem cell-derived mesenchymal stem cell, Normal healthy donors, PBMC peripheral blood mononuclear cell, PHA phytohemagglutinin, Treg regulatory T cells

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