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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients

Fig. 4

Role of PGE2 and cell–cell contact in iPSC-MSC-mediated immunomodulation. PBMC proliferation when cocultured with iPSC-MSCs and NS398 (a) or with iPSC-MSCs in transwell (b) determined by thymidine incorporation assay. Statistical analysis of activated T cells in lymphocytes when cocultured with iPSC-MSCs (c) or BM-MSCs (d) under different conditions determined by flow cytometry. e Statistical analysis of activated Treg cells in CD4+ cells when cocultured with iPSC-MSCs (e) or BM-MSCs (f) under different conditions determined by flow cytometry. g Statistical analysis of CD69+CD4+Foxp3+ cells in CD4+ cells when cocultured with iPSC-MSCs in transwell determined by flow cytometry. CD3+ T cells first gated. (n = 6 for each statistical analysis). *P < 0.05, **P < 0.01, ***P < 0.001 by Kruskal–Wallis rank-sum test followed by Mann–Whitney U test for two-group comparisons for (a), (b), and by one-way ANOVA and Dunnett T3 test for multiple comparisons for (c)–(g). 3H-TdR 3H-thymidine, AR allergic rhinitis, BM-MSC bone marrow-derived mesenchymal stem cell, cpm counts per minute, iPSC-MSC induced pluripotent stem cell-derived mesenchymal stem cell, Normal healthy donors, NS398 COX-2 inhibitor, PBMC peripheral blood mononuclear cell

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