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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients

Fig. 5

Role of NF-κB in iPSC-MSC-mediated immunomodulation. PBMCs from AR patients cocultured with shIKKβ-iPSC-MSCs. a qPCR analysis of IKKβ expression in iPSC-MSCs after lentiviral transduction (n = 6). b Flow cytometric analysis of activated CD4+ and CD8+ T cells in lymphocytes and Treg cells in CD4+ T cells with CD69+ cells when cocultured with shIKKβ-iPSC-MSCs. c Statistical analysis of activated CD4+ and CD8+ T cells in lymphocytes and Treg cells in CD4+ T cells when cocultured with shIKKβ-iPSC-MSCs (n = 6). d Flow cytometric and statistical analysis of CD69+Foxp3+CD4+ Treg cells in CD4+ T cells when cocultured with shIKKβ-iPSC-MSCs. CD3+ T cells first gated (n = 6). *P < 0.05, ***P < 0.001 by one-way ANOVA and Dunnett T3 test for multiple comparisons. iPSC-MSC induced pluripotent stem cell-derived mesenchymal stem cell, PBMC peripheral blood mononuclear cell, sh short hairpin

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Stem Cell Research & Therapy

ISSN: 1757-6512