Fig. 4

Isolation and characterization of adMSC-Exos. a Electron micrograph of exosomes isolated from adMSC-conditioned medium. The arrowheads indicate exosomes. Original scale bars = 0.5 μm. b Western blotting was performed with adMSC-Exos (Exos) or adMSC-conditioned medium (Medium). CD63, HSP70 and CD81 expression levels in exosomes were detected. c Fibroblasts were incubated with Dil-labeled exosomes (Dil-Exos; Dil is shown in red) or carrier control (CON), and nuclei were stained with Hoechst 33,342 (blue). Original scale bars = 50 μm