|
Group
|
Time point
|
Number of sheep
|
Surgery
|
BM aspiration
|
MION-labelled MSCs
|
Non-labelled MSCs
|
Gross assessment
|
MRI
|
Flow cytometery
|
Histological evaluation
|
|---|
|
Control (PBS)
|
Treated (MSC)
|
Total
|
|---|
|
1
|
1 day
|
0
|
2
|
2
| ● | ● | ● | | ● | ● | ● | ● |
|
2
|
1–2 week
|
1
|
3
|
4
| ● | ● | ● | | ● | ● | | ● |
|
3
|
4 weeks
|
6
|
6
|
12
| ● | ● | | ● | ● | | | ● |
|
4
|
12 weeks
|
8
|
8
|
16
| ● | ● | | ● | ● | | | ● |
|
5
|
24 weeks
|
8
|
8
|
16
| ● | ● | | ● | ● | | | ● |
- BM bone marrow, MION magnetic iron-oxide nanoparticle, MRI magnetic resonance imaging, MSC mesenchymal stem cell, PBS phosphate buffered saline, dots indicate procedures performed for each group
