Table 1 Quantitative real-time polymerase chain reaction primer list
Function | Gene | Forward primer | Reverse primer |
|---|---|---|---|
Hypoxia inducible | HIF1A | GGTCTAGGAAACTCAAAACCTGA | TCCTCACACGCAAATAGCTGA |
HIF2A | ATCAGCTTCCTGCGAACACA | GCTCCACCTGTGTAAGTCCC | |
ARNT | ACTACTGCCAACCCCGAAAT | CTCTGGACAATGGCTCCTCC | |
Notch pathway | HES1 | GTGTCAACACGACACCGGAT | GGAATGCCGCGAGCTATCTT |
HES3 | GATTTCCAAGCCGCTGATGG | TTCCGGATCTGGTGCGAGTA | |
HEY1 | TGCGGATTGAGCTAGTGCAT | AAGTAACCTTGGTCTCCCGT | |
HEY2 | GTGGGAAAGAGCCGCTAGG | GAGCTAGTACTTTGCCCCGA | |
Wnt/βcatenin pathway | AXIN2 | GCAACTCAGTAACAGCCCGA | CTCCTCTCTTTTACAGCAGGGC |
MMP7 | GTCTCTGGACGGCAGCTATG | GATAGTCCTGAGCCTGTTCCC | |
TCF-1 | CCAAGAATCCACCACAGAGACA | CAATGCCTATGGCTTCCTTGC | |
Hedgehog pathway | GLI1 | AGCCTTCAGCAATGCCAGTGAC | GTCAGGACCATGCACTGTCTTG |
PTCH1 | GCTGCACTACTTCAGAGACTGG | CACCAGGAGTTTGTAGGCAAGG | |
PTCH2 | GCACTATTACCGCAACTGGCTAC | TCTCCAGTCTGGATGAGCAGCT | |
SMO | AATGCGTGCTTCTTTGTGGG | TCTCATTGGAGGTGGGCTCC | |
House keeping | β-actin | AGAGCTACGAGCTGCCTGAC | AGCACTGTGTTGGCGTACAG |