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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A pivotal role of BEX1 in liver progenitor cell expansion in mice

Fig. 3

BEX1 promotes LPC proliferation in vitro. Primary LPCs isolated from 3-week CDE diet-fed WT and Bex1−/− mice. a Real-time PCR analysis of Bex1 expression in LPCs from WT and Bex1−/− mice (mean ± SEM, n = 5, **P < 0.01, Mann–Whitney U test). b Flow cytometry analysis of EpCAM, CD49f, CD44 and CD45 in LPCs from WT and Bex1−/− mice. Results representative of three independent experiments with similar results. c CCK8 assay showed proliferation of LPCs from WT and Bex1−/− mice (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). d Real-time PCR analysis of Myc and Cdkn1a expression in LPCs from WT and Bex1−/− mice (mean ± SEM, n = 5, **P < 0.01, Mann–Whitney U test). e Immunoblotting analysis of BEX1 expression in LEPCs infected with control lentivirus (shNC) or lentivirus expressing shRNA targeting BEX1 (shBex1).Quantifying densitometry of bands using ImageJ software (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). f CCK8 assay showed proliferation of shNC and shBex1 LEPCs (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). g BrdU assay showed proliferation of shNC andshBex1 LEPCs, and percentage of BrdU+ LEPCs analysed by flow cytometry (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). h Bex1 overexpressed in LEPCs. BEX1 expression detected by immunoblotting analysis. Quantifying densitometry of bands using ImageJ software (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). i CCK8 assay showed proliferation of Vector and Bex1 LEPCs (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). j BrdU assay showed proliferation of Vector and Bex1 LEPCs, and percentage of BrdU+ LEPCs analysed by flow cytometry (mean ± SEM, n = 4, *P < 0.05, Mann–Whitney U test). BEX1 brain-expressed X-linked 1, BrdU bromodeoxyuridine, EpCAM epithelial cell adhesion molecule, GAPDH glyceraldehyde 3-phosphate dehydrogenase, WT wild-type

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