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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Use of bioreactors for culturing human retinal organoids improves photoreceptor yields

Fig. 2

Photoreceptor quantification following bioreactor differentiation. a, b Cryosections from week 16 bioreactor cultures showing both CD73 and CD133 cell surface markers localised to ONL-like region (a) where RECOVERIN-positive photoreceptors are also located (b). c–e Representative FC analysis of week 16 bioreactor cultures vs control cultures: RECOVERIN-positive photoreceptor cells (68.17% ± 6.15 vs 43.53% ± 4.47) (c); CD133/CD73 double-positive developing rods (6.50% ± 0.86 vs 2.14% ± 0.57) (d); RECOVERIN/CD73 mature photoreceptors (11.87% ± 1.88 vs 1.38% ± 0.59) (e). Error bars, mean ± SEM; n = 50 retinal organoids per individual FC experiment, N = 3–4 independent differentiation experiments; *P < 0.05, **P < 0.01, two-tailed unpaired t test with Welch’s correction. Scale bars: 50 μM (a, b). ONL outer nuclear layer

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