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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Use of bioreactors for culturing human retinal organoids improves photoreceptor yields

Fig. 4

Bioreactor culture of retinal organoids increases cell proliferation and reduces cell death. a FC analysis of week 16 control vs bioreactor hPSC-derived retinal organoids stained with SYTOX Blue (error bars, mean ± SEM; n = 50 retinal organoids, N = 5 independent experiments; ns, P > 0.05, two-tail unpaired t test with Welch’s correction). b Immunohistochemistry analysis and quantification of cleaved caspase-3 retinal organoids showing reduction in number of caspase-3-positive apoptotic cells (error bars, mean ± SEM; n = 10, N = 3; *P < 0.05, two-tail unpaired t test with Welch’s correction). c Immunohistochemistry and quantification of Ki67 proliferative cells revealed significant increase in number of proliferating cells present in neuroepithelia of bioreactor cultures (error bars, mean ± SEM; n = 10 images, N = 3 independent experiments; *P < 0.05, two-tail unpaired t test with Welch’s correction). ns not significant

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