Fig. 7
From: Use of bioreactors for culturing human retinal organoids improves photoreceptor yields
Characterisation of human hPSC-derived neuroepithelia and photoreceptor cells at late stages of development. a Representative bright-field images of retinal organoids cultured in standard plates and bioreactors. a′ High-magnification images of neuroepithelia region showing CC/OS brush border in control but not in bioreactor samples. b Immunohistochemistry analysis for hMITOCHONDRIA and PRPH2 to delineate IS and OS, respectively, showed elongated OS-like structures in control samples only. c, c′ SEM images showing the topography of whole retinal organoids. Photoreceptor cell density and morphology from control vs bioreactor at ascending magnifications. Scale bars: 200 μM (a), 100 μM (a′), 25 μM (b), 20 μM (c), 10 μM (c′ and high magnification box). INL inner nuclear layer, IS inner segment, ONL outer nuclear layer, OS outer segment