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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Optimizing the method for generation of integration-free induced pluripotent stem cells from human peripheral blood

Fig. 3

Generation of integration-free iPSCs from PB MNCs with different episomal vectors. a Expression level of pluripotent genes 48 h after nucleofection by real-time PCR. CTRL group, PB MNCs before nucleofection. b Number of TRA-1-60-positive colonies generated from 1 × 106 PB MNCs 3 weeks after nucleofection. PB MNCs cultured for 8 days before nucleofection with episomal vectors (OS, MK) of different promoters (CAG, EF1, SFFV). PB MNCs (1 × 106 cells) nucleofected and then seeded into each well. c Number of TRA-1-60-positive colonies generated from 1 × 106 PB MNCs 3 weeks after nucleofection with different reprogramming factor-expressing episomal vectors. OS, pEV SFFV-OCT4-E2A-SOX2; MK, pEV SFFV-MYC-E2A-KLF4; Bcl-XL, pEV SFFV-BCL-XL; Shp53, pEV SFFV-Shp53. d Effect of c-MYC on generation of integration-free iPSCs from human PB. B, pEV SFFV-BCL2; K, pEV SFFV-KLF4. Data presented as mean ± SEM (n = 6) *P < 0.05, **P < 0.01, ***P < 0.001

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