Fig. 4

Characterization of integration-free iPSCs from PB MNCs. a Representative TRA-1-60 staining photograph of integration-free iPSC colony from PB MNCs. b Expression level of pluripotency genes of iPSCs compared with H1 by real-time PCR. c PB iPSCs expressed pluripotency markers OCT4, NANOG, TRA-1-60, and SSEA4. Representative images captured using Leica confocal microscope. d PB iPSCs formed teratoma in immunodeficient mice. H&E staining of representative teratoma from PB iPSCs with derivatives of three embryonic germ layers: cartilage (mesoderm), glands (endoderm), and neurotubules (ectoderm). e Representative karyotype of iPSC clone. All analyzed PB iPSC clones showed normal karyotype. f Vector sequence (EBNA1 and OSW) not found based on PCR-based detection in expanded iPSCs after 10 passages. MNC mononuclear cell, P passage