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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Inhibition of the RhoGTPase Cdc42 by ML141 enhances hepatocyte differentiation from human adipose-derived mesenchymal stem cells via the Wnt5a/PI3K/miR-122 pathway: impact of the age of the donor

Fig. 1

Cdc42 activity increases with age. hADSCs were isolated from lipoaspirates and cultured for 96 h and then treated for 24 h with or without the Cdc42/Rac1 GTPase inhibitor ML141 (ML; 10 μM). Cell lysates from undifferentiated hADSCs were used for the Pull-down assays of Cdc42-GTP (500 μg) and Cdc42GAP (100 μg). Correlations between donor age and Cdc42-GTP (a,e), donor age and Cdc42GAP (b,f), Cdc42-GTP and Cdc42GAP (c), Cdc42-GTP and cell yield/mg of collected adipose tissue (d), donor age and Rho-GTP (g), and donor age and Rac-GTP (h). Results represent the mean ± SEM of two independent experiments realized on the 61 subjects after normalization to GAPDH. Pearson correlation coefficient (R) and the P value are shown

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