Fig. 10

Global methylation status and involvement of DNA methyltransferases (DNMTs) in Hep-Dif of hADSCs. hADSCs derived from young and aged subjects were differentiated as indicated in Fig. 4. Cells were incubated with or without ML141 (ML, 10 μM) from day (D)−2 to D14 of the differentiation. a Methylation status of hADSCs derived from the young, aged, and ML141-treated aged groups were analyzed for the indicated time of Hep-Dif; expressions of DNMTs mRNA are shown. b hADSCs derived from aged subjects were subjected to Hep-Dif in the presence or absence of 5-azacytidine (AZA, 20 μM) for 48 h (from day 0 to day 2 of the differentiation)); ML141 was added from day −2 to day 14: methylation status, hepatocyte nuclear factor (HNF)4α mRNA, secreted albumin protein, and released exosomes are shown. Results are normalized as previously to the expression of the housekeeping gene and are the mean ± SEM of three independent experiments and are expressed as the percentage or fold variation of control relative to day D0. ^§*^¤P < 0.05, ^§§**^¤¤P < 0.01, ***P < 0.005; ^§aged vs young, *aged+ML vs aged, and ^¤AZA or AZA + ML vs control