Fig. 4

Impact of pharmacological targeting of Cdc42 activity by ML141 (ML) on the gene expression profile during Hep-Dif. hADSCs were isolated from young and aged subjects and were induced to Hep-Dif for 28 days with or without ML141 (10 μM) for the indicated time of incubation. RNAs were collected at D0/14/28 and mRNA levels of the studied genes were quantified by RT-qPCR. a Gene expression profile of hepatic and mesenchymal-to-epithelial transition markers as well as epigenetic markers (the DNA methyltransferases, DNMTs). b Rifampicin responsiveness of hepatocyte-like cells: RNAs were collected at day 28 of the differentiation after cell treatment with rifampicin (20 μM, 24 h). Expression levels of CYP3A4, CYP3A7, and albumin (ALB) genes are shown. The results are expressed as fold variation relative to controls (without rifampicin) after normalization to GAPDH, and are the mean ± SEM of two independent experiments performed twice, each in duplicate. D0 = day of induction of the differentiation. d-2/14, d14/28: cells were treated with ML141 from day −2 to day 14, or day 14 to day 28, respectively. ^§*P < 0.05, ^§§**P < 0.01, ***P < 0.001; ^§aged versus young or rifampicin versus basal and *aged treated with ML141 versus untreated