Fig. 1
From: Epithelial disruption: a new paradigm enabling human airway stem cell transplantation
LV-LacZ reporter gene expression at an MOI of 10 in (a) hABCs and (b) hAECs. c In vitro LacZ transduction efficiency for hABCs compared with hAECs. ****P < 0.0001, t test, n = 3. d Luciferase reporter gene expression (circular region of interest) in hAEC cultures after LV-Luc vector treatment at an MOI of 10 shows that the cells were successfully transduced prior to use in the in vivo studies. Scale bars = 20 μm. Abbreviations: hABC human airway basal cell, hAEC human amnion epithelial cell, LV-LacZ HIV-1–based LV vectors expressing nuclear-localised LacZ under transcriptional control of the MPSV promoter, MOI multiplicity of infection