Fig. 4

Treatments with Eph/Ephrin antagonists confirm that the blockade of Eph/Ephrin signaling results in increased cell death. To confirm the effects of soluble blocking of Eph/Ephrin-Fc fusion proteins on BM-MSC, cultures were established for 3 (gray columns) and 6 days (black columns) with different Eph/Ephrin antagonists. a At both time points, a blocking anti-EphrinB2 antibody, B11, produces higher proportions of apoptotic MSC than control ones supplied with Dulbecco’s modified Eagle’s medium (DMEM). b,c Likewise, small Eph/Ephrin blocking molecules, UniPR129 and UniPR500, affect MSC viability increasing the percentage of apoptotic BM-MSC 3 and 6 days after treatment as compared with control, dimethyl sulfoxide (DMSO)-treated cultures. Data were obtained from five different donors. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005, versus respective control