Fig. 4

Osteogenic gene and protein expression, and calcium deposition in BMSCs and ASCs. a and b Relative gene expression of the osteogenic genes Runx2, collagen type I, ALP and osteopontin in BMSCs and ASCs at day 7, 14 and 21, average of nine donors (a) and in each donor (b). c Western blot analysis of osteopontin in BMSCs and ASCs at day 21. d Representative images after Alizarin red S staining of BMSCs and ASCs at day 21 and 28. Scale bar 100 μm. e and f Quantification of Alizarin red S staining of BMSCs and ASCs at day 21 and 28, average of nine donors (e) and in each donor (f). Each symbol represents one donor. **p < 0.01, ***p < 0.001. ALP alkaline phosphatase, ASCs adipose-derived stem cells, BMSCs bone marrow-derived stem cells, Runx2 runt-related transcription factor 2