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Table 1 Antibodies

From: Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration

Terms

Source

Type

Company

Catalog number

Dilution

Thymosin beta 10 (TMSB10)

Rabbit

Polyclonal antibody

Abcam, USA

Ab14338

WB: 1:2000; IHC: 1:1000;

IF: 1:500 (whole antiserum)

Vascular endothelial growth factor-A (VEGFA)

Rabbit

Polyclonal antibody

Boster, China

BA0407

WB: 0.4 μg/ml (1:500)

Vascular endothelial growth factor-B (VEGFB)

Rabbit

Polyclonal antibody

Elabscience, China

E-AB-33212

WB: 1 μg/ml (1:1000)

Vascular endothelial growth factor-C (VEGFC)

Rabbit

Polyclonal antibody

Boster, China

BA0548

WB: 0.4 μg/ml (1:500)

Vascular endothelial growth factor-D (VEGFD)

Rabbit

Polyclonal antibody

Elabscience, China

E-AB-33213

WB: 1 μg/ml (1:1000)

Vascular endothelial growth receptor 1 (FLT1)

Rabbit

Polyclonal antibody

Proteintech, China

13687–1-AP

WB: 0.26 μg/ml (1:1000)

Vascular endothelial growth factor receptor 2 (KDR)

Rabbit

Polyclonal antibody

Bioss, China

Bs-10412R

WB: 2.5 μg/ml (1:400)

Vascular endothelial growth factor receptor 3 (FLT4)

Rabbit

Polyclonal antibody

Proteintech, China

20712–1-AP

WB: 0.83 μg/ml (1:500)

Rabbit IgG-Isotype control

Rabbit

 

Abcam, USA

Ab172730

IHC: 1.78 μg/ml (1:1000)

IF: 3.56 μg/ml (1:500)

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH)

Mouse

Monoclonal antibody

Proteintech, China

60004–1-Ig

WB: 0.5 μg/ml (1:2000)

HRP-conjugated goat anti-rabbit IgG (H + L)

  

Beyotime, China

A0208

WB: 1:2000

IHC: 1:500

HRP-conjugated goat anti-mouse IgG (H + L)

  

Beyotime, China

A0216

WB: 1:2000

Alexa Fluor 488 labeled goat anti-rabbit IgG (H + L)

  

GeneCopoeia, USA

L110B

IF: 1:250

  1. HRP horseradish peroxidase, IF immunofluorescence, IHC immunohistochemistry, WB Western blotting