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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis

Fig. 4

Validation and genotyping of equine keratinocyte cell lines. (a, b) PCR amplification with equine MT-ATP6/MT-ATP8 (top)and GAPDH (bottom) primers; NTC – no template control, HFK - human foreskin keratinocytes, K9 - dog cells, J2 - mouse fibroblasts, EK100 - equine keratinocytes, EK1547- equine keratinocytes; (c) FISH with BACs 49H16 (chr9, green) and 76H13 (chr29, red) in EK1547; (d) Cytogenetic analysis of EK1547: G-banded metaphase (left), G-banded karyotype (middle), C-banded metaphase (right); (e) Cytogenetic analysis of EK100: Giemsa stained metaphase (left), Giemsa stained karyotype (middle), C-banded metaphase (right)

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Stem Cell Research & Therapy

ISSN: 1757-6512