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Table 1 Culture conditions were used for growing equine keratinocytes

From: Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis

Growth media Calcium
concentrationa
(mM)
Y-27632
concentration
(uM)
FBS
concentration
Survival Ability to passage
KSFM+ suppa 0.1 No No serum No No
KSFM+ suppa 0.1 Yes No serum No No
CnT-09+ suppb 1.8 No 10% yes No
CnT-09+ suppb 1.8 Yes 10% Yes Yes
Fmedia 1.36 No 7.40% Yes Yes (1 passage)
Fmedia 1.36 Yes 7.40% Yes Yes
Fmedia
+
irradiated feeders
1.36 Yes 7.40% Yes Yes
  1. suppa Epidermal growth factor 1–53 (EGF 1–53) and bovine pituitary extract (BPE)
  2. suppb Supplements A and Supplements B (200 mM L-glutamine)
  3. aCalcium concentration in FBS is not included