Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis

Table 1 Culture conditions were used for growing equine keratinocytes

Growth media	Calcium concentration^a (mM)	Y-27632 concentration (uM)	FBS concentration	Survival	Ability to passage
KSFM+ ^suppa	0.1	No	No serum	No	No
KSFM+ ^suppa	0.1	Yes	No serum	No	No
CnT-09+ ^suppb	1.8	No	10%	yes	No
CnT-09+ ^suppb	1.8	Yes	10%	Yes	Yes
Fmedia	1.36	No	7.40%	Yes	Yes (1 passage)
Fmedia	1.36	Yes	7.40%	Yes	Yes
Fmedia + irradiated feeders	1.36	Yes	7.40%	Yes	Yes

suppa Epidermal growth factor 1–53 (EGF 1–53) and bovine pituitary extract (BPE)
suppb Supplements A and Supplements B (200 mM L-glutamine)
^aCalcium concentration in FBS is not included

ISSN: 1757-6512