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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products

Fig. 3

HBV DNA detection in HUMSCs after incubation with human sera with HBV using FQ-PCR. a HBV DNA detection in HUMSCs without medium change and passage after incubation with human sera from HBV-infected donor. b HBV DNA detection in HUMSCs with medium change every 2 days. HBV DNA levels gradually decreased in cell lysate and medium from day 0 to 8. c HBV DNA detection in HUMSCs with passage. In cell lysate, HBV DNA level in P3 HUMSCs significantly higher that than in P4 HUMSCs, and failed to be detected in P5–P7 HUMSCs. In culture medium, HBV DNA detected only in P3. HBV hepatitis B virus, * represents p < 0.05 and p < 0.05 was considered statistically significant

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Stem Cell Research & Therapy

ISSN: 1757-6512