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Table 3 Detection limit of ddPCR for HBV DNA

From: Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products

Dilution

Concentration (copies/μl)

Self-designed primer probe

Commercial primer probe

1×

3710

3246

10×

402

343

102×

42.8

32.9

103×

4.1

3.9

104×

0.58

0.21

105×

0.06

0

106×

0

0.06

107×

0

0

  1. ddPCR droplet digital PCR, HBV hepatitis B virus