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Table 4 Failed detection of HBV DNA in culture medium and cell lysate of MSCs derived from HBV-infected donors using ddPCR

From: Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products

Donor

 

Concentration (copies/μl)

Self-designed primer probe

Commercial primer probe

No. 1

3rd passage medium

0

0

3rd passage cell lysate

0

0

No. 2

3rd passage medium

0

0

3rd passage cell lysate

0

0

No. 3

3rd passage medium

0

0

3rd passage cell lysate

0

0

  1. HBV hepatitis B virus, MSC mesenchymal stem cell, ddPCR droplet digital PCR