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Table 4 Failed detection of HBV DNA in culture medium and cell lysate of MSCs derived from HBV-infected donors using ddPCR

From: Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products

Donor   Concentration (copies/μl)
Self-designed primer probe Commercial primer probe
No. 1 3rd passage medium 0 0
3rd passage cell lysate 0 0
No. 2 3rd passage medium 0 0
3rd passage cell lysate 0 0
No. 3 3rd passage medium 0 0
3rd passage cell lysate 0 0
  1. HBV hepatitis B virus, MSC mesenchymal stem cell, ddPCR droplet digital PCR