Fig. 2
From: Exosomes from acellular Wharton’s jelly of the human umbilical cord promotes skin wound healing
Analysis of exosome interaction with fibroblast cells Exosomes were labeled with CD81 antibody and treated with gold nanoparticles for visualization. a Transmission electron microscopy (TEM) at 7.8k× magnification showing an entire cell. b, c 100k× magnification of the cell focusing on exosomes surrounding the cell. Black arrows point to exosomes. d Red fluorescently labeled exosomes concentrate to the green EYFP fibroblast cells and over a period of 2.5 h, the red fluorescence starts to diminish. e Control green EYFP cells tracked over time without any exosomes. f Magnified images at 30 min, 90 min (1.5 h), and 180 min (3 h) displaying a gradual decrease in red fluorescence and a complete lack of red fluorescence by 3 h. N = 3