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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Autologous transplantation of adipose-derived stem cells improves functional recovery of skeletal muscle without direct participation in new myofiber formation

Fig. 1

Effect of adipose-derived stem cells (ADSC) on satellite cell (SC) activation and differentiation. a Relative mRNA level of paired box protein domain 7 (Pax7) and myogenic differentiation antigen (MyoD) transcription factors in SC cocultured with/without ADSC in proliferation-induction medium. b Protein expression of Pax7 and MyoD (upper band) transcription factors in SC cocultured with/without ADSC in proliferation-induction medium. c Relative mRNA level of Pax7 and MyoD expressed in SC in proliferation-induction ADSC conditioned medium (CM-ADSC). d Relative mRNA level of MyoD and MyHC in MPC cocultured in differentiation-inducing medium with/without ADSC. e Relative protein expression of MyoD and MyHC in MPC in differentiation medium cocultured with/without ADSC and relative mRNA levels of expression of MyoD and MyHC in a differentiation-inducing conditioned medium from ADSC. f Relative mRNA levels of MyoD and MyHC expressed in MPC in differentiation-induction CM-ADSC. g Average number of multinucleated fibers per high-power filed (HPF) between MPC cocultured with/without ADSC, shown as mean ± SD. The relative level of mRNA was measured using RT-qPCR and the relative protein level was determined with Western blot. All the results were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression

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Stem Cell Research & Therapy

ISSN: 1757-6512