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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

Fig. 2

Characterization of BMSCs and ADSCs using (a) flow cytometry for positive markers such as CD29, CD73, CD90, CD105, and HLA-I and negative markers such as HLA-II, CD34, and CD45. b Tri-lineage differentiation potential. (i) Osteogenesis was determined by Alizarin red S staining of the extracellular mineralized matrix. (ii) Adipogenesis was determined by using Oil red O staining of lipid droplets. (iii) Chondrogenesis was determined by Alcian blue staining of proteoglycan. All images were taken at 20× magnification. Abbreviations: ADSC adipose tissue–derived mesenchymal stem cell, BMSC bone marrow–derived mesenchymal stem cell

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