Fig. 2From: An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cellsCharacterization of BMSCs and ADSCs using (a) flow cytometry for positive markers such as CD29, CD73, CD90, CD105, and HLA-I and negative markers such as HLA-II, CD34, and CD45. b Tri-lineage differentiation potential. (i) Osteogenesis was determined by Alizarin red S staining of the extracellular mineralized matrix. (ii) Adipogenesis was determined by using Oil red O staining of lipid droplets. (iii) Chondrogenesis was determined by Alcian blue staining of proteoglycan. All images were taken at 20× magnification. Abbreviations: ADSC adipose tissue–derived mesenchymal stem cell, BMSC bone marrow–derived mesenchymal stem cellBack to article page