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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

Fig. 4

Transmission electron microscopic (TEM) pictures of exosomes isolated by hMSCs by using the UC and SUC methods. a Representative images depicting a higher number of exosomes in the SUC method for both tissuespecific hMSCs. b & c Morphometric evaluation of exosome by further diluting the samples for shape, size, and number. Exosomes isolated by the SUC method were cup-shaped and greater in number compared with those isolated by the UC method. Also, small-sized vesicles (<30 nm) were observed to be in clusters of four to five vesicles each. Red circle depicts grouped or coupled vesicles Yellow circle depicts individual vesicles. All images were taken at 15,000× magnification and for morphological analyses images were magnified at 20,000× magnification. Abbreviations: hADSC human adipose tissue–derived mesenchymal stem cell, hBMSC human bone marrow–derived mesenchymal stem cell, hMSC human mesenchymal stem cell, ns non-significant, SUC one-step sucrose cushion ultracentrifugation, UC direct ultracentrifugation

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