Fig. 4From: An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cellsTransmission electron microscopic (TEM) pictures of exosomes isolated by hMSCs by using the UC and SUC methods. a Representative images depicting a higher number of exosomes in the SUC method for both tissuespecific hMSCs. b & c Morphometric evaluation of exosome by further diluting the samples for shape, size, and number. Exosomes isolated by the SUC method were cup-shaped and greater in number compared with those isolated by the UC method. Also, small-sized vesicles (<30 nm) were observed to be in clusters of four to five vesicles each. Red circle depicts grouped or coupled vesicles Yellow circle depicts individual vesicles. All images were taken at 15,000× magnification and for morphological analyses images were magnified at 20,000× magnification. Abbreviations: hADSC human adipose tissue–derived mesenchymal stem cell, hBMSC human bone marrow–derived mesenchymal stem cell, hMSC human mesenchymal stem cell, ns non-significant, SUC one-step sucrose cushion ultracentrifugation, UC direct ultracentrifugationBack to article page