Skip to main content
Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Periosteal progenitors contribute to load-induced bone formation in adult mice and require primary cilia to sense mechanical stimulation

Fig. 4

Periosteal OCPs uniquely respond to mechanical stimulation in an osteogenic manner. Primary periosteal cells isolated from 3-week-old Prx1CreER-GFP mice were exposed to 1 h of oscillatory fluid flow (OFF) and changes in mRNA expression were quantified via RT-qPCR. Prx1-expressing OCPs (green) exposed to OFF (line pattern) demonstrated increases in Cyclooxygenase-2 (COX-2) (a) and Osteopontin (OPN) expression (b) compared with controls, whereas other cells of the periosteum (white) were nonresponsive. OCPs have elevated OPN expression compared with other cells of the periosteum under static conditions (b). Additionally, the fold changes in COX-2 (c) and OPN (d) expression were significantly higher for OCPs compared with other cells of the periosteum. mRNA expression was normalized to GAPDH expression. Data are reported as mean and standard error. Other no-flow n = 5, other flow n = 7, OCP no-flow and flow n = 6, *p < 0.01, ***p < 0.0001

Back to article page