Fig. 5
OCP primary cilia are necessary for the flow-induced osteogenic response. Primary periosteal OCPs isolated from 3-week-old Prx1CreER-GFP;Ift88fl/fl mice were treated with the active compound of tamoxifen or vehicle control and exposed to 1 h of oscillatory fluid flow (OFF). Vehicle control OCPs (white) demonstrated an increase in Cyclooxygenase-2 (COX-2) (a), Osteopontin (OPN) (b), and Runt-related transcription factor 2 (RUNX2) (c) expression, but a decrease in Bone gamma-carboxyglutamic acid-containing protein (BGLAP) (d) expression. These effects were lost in OCPs treated with tamoxifen (gray) to disrupt ciliogenesis. mRNA expression was normalized to GAPDH expression and OFF samples were normalized to static controls. Data are reported as mean and standard error. Vehicle n = 6, tamoxifen n = 5, ***p < 0.0001