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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Biphasic modulation of insulin signaling enables highly efficient hematopoietic differentiation from human pluripotent stem cells

Fig. 1

Insulin withdrawal promotes vascular mesoderm differentiation. a Schematic view of monolayer-based mesoderm differentiation from hPSCs. Representative flow cytometry results showing proportion of FLK1+ cells at differentiating day 3. b RT-qPCR analysis of pluripotency (OCT4, NANOG), mesendoderm (T, MIXL1), mesoderm (MESP1, KDR, MEIS1, MYB, PDGFRa) and vascular progenitor cell genes (ETV2) at differentiating day 3. N = 3 independent replicates. Error bars represent mean ± SEM. n.s. not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. c Representative flow cytometry results of vascular progenitor marker FLK1, pan-mesoderm surface marker CD56 and CD13 at differentiating day 3. d Statistical analysis of flow cytometry results at differentiating day 3. N = 3 independent replicates. Error bars represent mean ± SEM. n.s. not significant, *P < 0.05, **P < 0.01. e Representative morphology of differentiating cells from day 1 to day 3. Scale bars, 50 μm. f Growth kinetics of differentiating cells under different conditions as indicated from day 1 to day 3. N = 3 independent replicates. Error bars represent mean ± SEM. **P < 0.01 ***P < 0.001. FACS fluorescence-activated cell sorting, hESC human embryonic stem cell, hPSC human pluripotent stem cell, ME mesoderm, Rap rapamycin, RT-qPCR reverse transcriptase quantitative PCR

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