Fig. 6

Characterization of HSPC-derived macrophages in vitro. a Schematic strategy for generating macrophages from HSPCs. b Morphology change during macrophage differentiation assessed by light microscopy and Giemsa staining. Scale bars, 25 μm. c Growth kinetics of differentiating iMac in 1 week (n = 3 independent experiments, mean ± SEM). d Surface marker expression of CD45, CD43, CD34, CD11b, CD14, CD68 and CD163 on H1-Mac analyzed by flow cytometry. e Phagocytosis test of differentiated macrophages. Arrows point to several GFP-C. albicans engulfed by a macrophage. Scale bars, 10 μm. f Flow cytometry analysis of cells’ ability to engulf GFP-C. albicans. Control cells: undifferentiated H1 ESCs. g Flow cytometry analysis of cells’ ability to engulf GFP-labeled M. bovis-BCG and M. abscessus. Negative control cells: UC-MSCs. h Cytokine gene expression of UC-MSCs and H1-Mac upon stimulation by LPS (mean ± SEM, n = 3). i Zika viral antigen and cytokine expression in iMac 4 days after virus infection (mean ± SEM, n = 3). ESC embryonic stem cell, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HSPC hematopoietic stem progenitor cell, IL interleukin, iMac induced macrophages, LPS lipopolysaccharide, M-CSF macrophage colony-stimulating factor, TNFa tumor necrosis factor alpha, UC-MSC umbilical cord mesenchymal stem cell, BF bright field, GFP green fluorescence protein