Fig. 1

High DEPTOR levels associated with reduced bone formation in vivo. a Significantly enriched disease terms (top 10) associated with DEPTOR knockdown. b H&E staining of femurs at distal metaphysis growth plate area in Sham and OVX mice (N = 6). Scale bars: 200 μm. c μCT images of femurs at distal metaphysis growth plate area in Sham and OVX mice (N = 6). Scale bars: 1 mm. d Bone volume to total volume (BV/TV), trabecular bone thickness (Tb.Th), and trabecular bone number (Tb.N) reduced in OVX mice (N = 6). e Deptor expression of Sham and OVX mice evaluated by histology and immunofluorescence (N = 6). Scale bars: 50 μm. f Deptor expression of Sham and OVX mice evaluated by IHC (N = 6). Arrows indicate Deptor-positive areas. Scale bars: 100 μm. g Relative mRNA expression of Deptor and Ocn by qRT-PCR in BMSCs isolated from Sham and OVX mice. Gapdh used for normalization. h Left: western blot analysis of Deptor and Ocn protein levels in BMSCs isolated from Sham and OVX mice. Gapdh used as internal control. Right: quantification of band intensities. Data presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 3 independent experiments). See Additional file 2: Figure S1. DAPI 2-(4-amidinophenyl)-1H-indole-6-carboxamidine, DEPTOR DEP domain containing mTOR interacting protein, GAPDH glyceraldehyde 3-phosphate dehydrogenase, Ocn osteocalcin, OVX ovariectomy