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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis

Fig. 2

DEPTOR is downregulated in osteogenic differentiation of hBMSCs. a Relative expression of DEPTOR during osteogenic differentiation of hBMSCs determined by qRT-PCR. GAPDH used for normalization. b–d Relative mRNA levels of osteogenic markers (b) RUNX2, (c) ALP, and (d) OCN during osteogenic differentiation of hBMSCs, determined by qRT-PCR. GAPDH used for normalization. e Western blot analysis of DEPTOR and RUNX2 protein levels during osteogenic differentiation of hBMSCs. GAPDH used as internal control. Data presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, NS not significant (n = 3 independent experiments). ALP alkaline phosphatase, d day, DEPTOR DEP domain containing mTOR interacting protein, GAPDH glyceraldehyde 3-phosphate dehydrogenase, Ocn osteocalcin, RUNX2 runt related transcription factor 2

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Stem Cell Research & Therapy

ISSN: 1757-6512