Fig. 1

Effect of conditioned medium from different cell types on platelet activation. Platelets incubated with either cell culture or conditioned medium (CM) and then stimulated by TRAP-6. Platelet activation measured by flow cytometry, assessing expression of CD62P, CD63 and PAC-1 binding. a Bone marrow (BM)-MSCs. b Lipoaspirate (LA)-MSCs. c Cord blood (CB)-MSCs. d Human umbilical vein endothelial cells (HUVECs). e HeLa tumor cells. Scale indicates relative activation marker expression of CM compared to culture medium. n = 3 biological replicates; HeLa cells n = 1, note different y axis for HeLa cells. *p < 0.05, **p < 0.01, ***p < 0.001. MFI mean fluorescence intensity