Fig. 2
Effect of different cell types and dosages on platelet activation and resting platelets in flow cytometry. Platelets incubated with or without (w/o) indicated cell types. Then platelets either activated by TRAP-6 or remained resting. a, b Influence of 5 × 105 cells/ml on a TRAP-6-induced platelet activation and b resting platelets. Mean fluorescence intensity (MFI) of different thrombocyte activation markers assessed: CD62P, PAC-1 binding and CD63 (n = 8–17). c, d Dose effect of different cell types on CD62P detection on c TRAP-6-stimulated platelets and d resting platelets (n = 5–6). Different MFI values of platelets explained by different platelet donors used in individual experiments. In all experiments, paired analysis against control w/o cells was performed. *p < 0.05, **p < 0.01, ***p < 0.001. BM bone marrow, CB cord blood, HUVEC human umbilical vein endothelial cell, LA lipoaspirate