Fig. 4

Multi-potential differentiation of NPSCs. Osteogenic differentiation at 3 weeks. Mineralization was positive for Alizarin Red staining (a). Adipogenic differentiation at 2 weeks. Secretion of oil droplets were positive for Oil Red O staining (b). Chondrogenic differentiation at 3 weeks. Cells were stained with Alcian Blue staining (c). Scale bars, 100 mm. L-PRP leukocyte-platelet-rich plasma, NPSCs nucleus pulposus-derived stem cells, P-PRP pure platelet-rich plasma