Table 1 Primers used in polymerase chain reaction and quantitative real-time polymerase chain reaction for gene expression analysis
Gene | Primer sequence (5′to3′) |
|---|---|
CD29-F | GTCACCAACCGTAGCAA |
CD29-R | CTCCTCATCTCATTCATCAG |
CD44-F | CGATTTGAATATAACCTGCCGC |
CD44-R | CGTGCCCTTCTATGAACCCA |
CD166-F | GGACAGCCCGAAGGAATACGAA |
CD166-R | GACACAGGCAGGGAATCACCAA |
CD4-F | GATGGAGGTGGAACTGC |
CD4-R | GGAAAGCCCAACACTATG |
CD8-F | GGGTGGAAAAGGAGAAGC |
CD8-R | AGGTGAGTGCGGGAGAC |
CD14-F | CAGGTGCCTAAGGGACT |
CD14-R | AATAAAGTGGGAAGCGG |
Coll II-F | CAGGATGTCCAGGAGGCT |
Coll II-R | GCAGTGGCGAGGTCAGTAG |
AGC-F | GGAGCCCGAGCCTATACTATTT |
AGC-R | CCCAAGGACCACCAATCA |
IL-6-F | AGGGAGGTCGAGCTGTTCTC |
IL-6-R | GGAGTGTTCACTAAGCGGTCA |
IL-1β-F | CGGTCAAGGAGAGGAGCTTAC |
IL-1β-R | GGACTAGCCCTCGCTTATCTTT |
TNF-α-F | GGAGAAGCCGGTAGTGGAGAT |
TNF-α-R | GGTCTGGTCACGGTTTGGAA |
IL-8-F | CACAGTGGACGACATCCGAAA |
IL-8-R | AGCTACATAGGAATTACGGGCAA |
MMP-1-F | CGACTCGCTATCTCCAAGTGA |
MMP-1-R | GTTGAACCAGTCTCCGACCA |
MMP-13-F | GGAGGCGAGAACATCAAGCC |
MMP-13-R | CGGCCTTCCCTCGTAGTGA |
OCT4-F | ACCTTCATCGGAAACTCCAAAG |
OCT4-R | ACTGTTAGGCTCAGGTGAACT |
Nanog-F | CTGTGGGTTTCTGTGCTGG |
Nanog-R | CCGGCTTCAAGGCTTTCAG |
GAPDH-F | ACTTTGTGAAGCTCATTTCCTGGTA |
GAPDH-R | GTGGTTTGAGGGCTCTTACTCCTT |