Fig. 2

The effect of ASC-exosomes on the level of serum IgE, the number of eosinophils, and the expression of pro-inflammatory cytokines in NC/Nga mice. a The level of serum IgE was detected by ELISA. b The number of eosinophils in blood was determined by differential cell counting. c–f Total RNA was isolated and the mRNA levels of IL-4 (c), IL-31 (d), TNF-α (e), and IL-23 (f) were detected by quantitative real-time PCR and normalized by GAPDH mRNA expression. Results are presented as mean ± standard error of the mean; n = 10. *P < 0.05, **P < 0.01, and ***P < 0.001 vs vehicle group. IV intravenous administration, SC subcutaneous administration, Pred prednisolone