Fig. 2From: Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2PCR screening for validation of selected clones. PCR primers flanking PRF1 gene exon 2 designed to yield a 1.3-kb amplicon in nontargeted (wild-type) genome. Homozygous insertion of an expression cassette containing human ubiquitin C promoter driving the puromycin resistance gene yields a 2-kb amplicon (a). Analysis of PCR results on 1% agarose gel confirmed homozygous 2-kb insertion into PRF1 exon 2 in selected NK92 clones (b). Donor plasmid vector used as positive control and human control DNA as negative controlBack to article page