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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2

Fig. 2

PCR screening for validation of selected clones. PCR primers flanking PRF1 gene exon 2 designed to yield a 1.3-kb amplicon in nontargeted (wild-type) genome. Homozygous insertion of an expression cassette containing human ubiquitin C promoter driving the puromycin resistance gene yields a 2-kb amplicon (a). Analysis of PCR results on 1% agarose gel confirmed homozygous 2-kb insertion into PRF1 exon 2 in selected NK92 clones (b). Donor plasmid vector used as positive control and human control DNA as negative control

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Stem Cell Research & Therapy

ISSN: 1757-6512